Electrophoresis is a technique that allows us to separate DNA, RNA or proteins according to their size. 

In this video, we show you how to prepare an agarose gel for electrophoresis

1. DILUTE concentrated (50X) buffer with distilled water to create 1X buffer.

2. MIX agarose powder with 1X buffer in a 250 ml flask.

3. DISSOLVE agarose powder by boiling the solution. Microwave the solution on high for 1 minute. Carefully remove the flask from the microwave and mix by swirling the flask. Continue to heat the solution in 15-second bursts until the agarose is completely dissolved (the solution should be clear like water).

4. COOL agarose to 60° C with careful swirling to promote even dissipation of heat.

5. While agarose is cooling, Seal the ends of the gel-casting tray with the rubber end caps. Place the well template (comb) in the appropriate notch.

6. POUR the cooled agarose solution into the prepared gel-casting tray. The gel should thoroughly solidify within 20 minutes. The gel will stiffen and become less transparent as it solidifies.

7. REMOVE end caps and comb. Take particular care when removing the comb to prevent damage to the wells.

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